How the Zergs were Made

Chapter 4: surgery

   First of all, it is to untwist first, to untie the messy inner core.

   Soon, the single-cell DNA strands were unreservedly presented in front of Huo Gu.

   Next is ‘Intercept’, cutting out that part of the gene chain expressing the phage. This is a very delicate task, especially for Huogu, who now has no hands or feet.

Carefully control the enzyme's output restriction endonucleases to ensure that other parts of the gene chain are not damaged. At the same time, rely on instructions to remotely control single cells and use cytoplasmic flow to limit the endonucleases to the gene fragments that need to be excised. .

  This kind of behavior is tantamount to writing with saliva and writing on the water.

   "Oops!"

With the emergence of Huogu’s chagrin, the wrong endonuclease was cut all the way, and other gene fragments were also cut. The single-cell core shrank by nearly half in a short while, until the inner Exhaustion of Dicer.

   Then, in the ligase secreted by the single cell instinctively, these fragmented gene fragments are joined to each other and once again combined into a complete gene chain.

   However, Huo Gu knew that it was no longer possible to replicate the original single cell with this greatly altered gene chain.

   Huogu re-selected new cells in the single-cell cluster as the target of the ‘surgery’.

   The failure just now did not deal much blow to Huogu's confidence. It never considered itself a genius, and even felt that it was a little stupid than the average person, but read a little more books than others.

   The operation restarted.

   This time, after learning the lessons from the previous one, Hogu used the endonucleases in the single cells more carefully, greatly reducing the amount of endonucleases, and most of his attention was focused on the movement of the cytoplasm.

   Let the cytoplasm flow and guide those endonucleases to cut out the designated gene fragments. At the same time, after the cut, it just runs out.

  Failed...

  Failed...

  Failed...

  ...

  Failed...

   Success!

"that's good."

   Just as Huo Gu firmly believed, it succeeded. After nearly a hundred cells were lost, the gene fragment of the phage was cut out.

   The next step is to connect. This part is relatively simple. Huogu controls the cell remotely and uses the flow of cytoplasm to restrict the phage-expressing gene fragment to a certain area.

   Then, relying on ligase, the DNA that had been divided into two segments after the operation was recombined into one, forming a brand new gene chain.

   "began to split"

   Huogu commanded this, and the replica cells that had undergone successful surgery began to gradually divide into new individuals.

   Soon, a new individual with a single-cell cluster appeared, but this new individual still showed some unexpected conditions.

   That is the "cell wall".

   The replica cell is surrounded by a shell. Compared with the cell membrane, this shell is stronger and has a certain degree of elasticity, which can give the protective cell sufficient growth space.

"This…"

   "Why?"

   Huo Gu thought for a while, not very sure.

   "Is it the result of the incomplete phage gene expression?"

   The operation did not completely cut the gene of the phage. Huogu only removed the part of the gene fragment that was previously known to express the toxic phage.

   Anyway, incomplete gene expression is also incomplete, and it is impossible to transcribe phage.

   For the sake of perfect removal, risking the reappearance of toxic phage?

   At that time, Huo Gu thought about it, but forget it, he is not a perfectionist, there is no need to do that kind of death.

   I didn’t expect this to happen...

   The cell wall must have, because the main function of the cell wall is to protect the cell.

   A simple understanding is that the commoner Diaomin puts on the steel armor, and the defense power is not the same as before.

   Originally, Huogu planned to start the production of cell walls after solving the phage problem, but now...

   Huogu fell into entanglement.

   is not to say that it doesn't like such surprises, but after all, this is the cell wall obtained by ‘accidentally’. It’s really hard to say whether it can meet the requirements it wants.

   "It's decided, let's observe it for a while."

   Hogu made up his mind like this, his attention shifted to the other single cells and started the operation.

   With the first successful case, there will naturally be a second and a third time. After a while, Huogu has already practiced and made perfect, and the success rate of cutting gene fragments has stabilized at more than 80%.

   During this period, Huogu thought of a way to break the protein structure connection, which is to rely on division to create a new replica between two connected single cells and squeeze out the old single cells.

   This is somewhat similar to the principle of The Ship of Theseus. Without affecting normal thinking, gradually replace those old single cells.

   Huo Gu, who thought so, did indeed do so.

   As the last infected single cell is replaced, the sense of crisis disappears, UU reading www.uukanshu. Com replaces it with an incomparable sense of peace of mind. After all, things that can threaten it are temporarily gone.

   Set up a good mood, Huo Gu did not forget his current thinking carrier, but a group of insignificant single-cell clusters, a lower level of life than the bottom plant of the food chain.

   Huo Gu thought that when it was still a human, it had at least 100 million ways to obliterate the current single-cell clusters, and when its thoughts drifted here, it shuddered.

   "Too fragile, I still need to continue to optimize these single cells."

Hogu, who established his goal, began to analyze the unexpected surprises. If the effect of these cell walls is not satisfactory, then it is necessary to undergo surgery to completely remove the phage gene fragments, and then rebuild the ones that can meet the needs. Cell wall.

The first requirement is proper permeability. If the permeability is too good, the protective effect will be minimal. If the permeability is too poor, there will be less external substances, and the cells will gradually decline. .

The second requirement is proper flexibility. The cell wall must not be too hard or too soft. It must also have a certain degree of ductility. Too hard will limit the growth of the cell itself, and too soft will not have a buffer protection effect and cannot be stretched. In this case, the plasticity is also very poor, which is not conducive to huddling together in this environment.

   The third requirement is what Huogu needs most urgently at present, and that is to be able to defend against the attacks of bacteriophages, aggressive bacteria, and fungi lurking in the dark.

The first and second requirements are easy to test. You only need to place a single cell alone at the outermost periphery. The rest of the work is just to observe to see if the single cell used as the experimental object has changed, and to what extent. .

   The real difficulty is the third.