"Old Su, we don't have enough protein here." Fan Zhenlong walked around from the side of the experimental table, yelled, and then retracted his head to get busy.

Su Xiankai hummed twice and complained: "Why are you running out again?"

"No way, I can't get results all the time." Fan Zhenlong said annoyedly: "The amount of protein on our side has exceeded the plan."

Su Xiankai's eyebrows twitched twice and said, "I'm not sure I can make the planned amount. If you exceed the plan, what should I do?"

Fan Zhenlong said helplessly: "Of course we don't want to go beyond the plan... We can only say that our previous thinking was too simple. The three-dimensional structure of this thing is much more difficult than expected."

"It's really difficult." Gu Qiang was reluctant to admit it, but he still said, "Our original plan might have to be completely revised..."

"No need to modify." Yang Rui himself kept scanning the literature, and said: "The newly ordered equipment will be here soon. Besides, Lao Su, I will add two more people to you."

When Yang Rui made the plan, he knew that he was going to overspend by a large margin, and he was well prepared for everything.

He spent 6 million U.S. dollars, and spent half of the budget. The rest is for the reserve team. Moreover, he still has a large sum of RMB that has not been used. The funds for the ion channel laboratory, plus the 863 plan, It's not a problem to make up three million yuan.

If that is not enough, he estimates that he can get another sum of money from Peking University. In theory, a large amount of donations can be raised from the domestic biomedical and animal husbandry central enterprises and even the ministries and commissions.

The advantage of brushing up reputation lies in this. As long as the project is successful, these donations will be taken for nothing.

As for the failure of the project, the loss is the reputation itself.

Yang Rui actually doesn't know how much money is needed to achieve the desired results, but he knows how much foreigners use. Therefore, he has a considerable estimate of the difficulty of each link.

As soon as Su Xiankai's head turned, he knew that Yang Rui had been prepared, which made him a little embarrassed.

Yang Rui saw it, and Su Xiankai was embarrassed for a moment, and said: "The plan we made is based on a foreign first-class laboratory. Looking at it now, we have not yet reached the level of a foreign first-class laboratory. It doesn't matter, I More prepared funds and more prepared resources are meant to bridge the gap between us."

After a pause, Yang Rui said again: "G protein-coupled receptor is a world-class project. After all, it will be bayonet with foreign first-class laboratories. Therefore, including me, everyone must grow up as soon as possible."

When he said this, Su Xiankai's expression eased.

However, the pressure of reality quickly pressed on Su Xiankai's body.

"Just adding two people and a batch of machines can't make up the gap between us and foreign first-class laboratories." Su Xiankai noticed a loophole in Yang Rui's logic.

Yang Rui curled his lips and said: "If you do a project like a foreign first-class laboratory, it will definitely not work, but our goal is to concentrate the material on bovine rhodopsin protein."

Several people in the laboratory looked at Yang Rui without knowing it.

Su Xiankai even asked, "Isn't our material just bovine rhodopsin protein?"

"We are, but this is not necessarily the case in foreign laboratories."

"why?"

"Because they want better materials and want to try more templates. This is our opportunity." Yang Rui didn't say a word, that is, he knew that at this stage, bovine rhodopsin protein is the best. The material too.

The g protein-coupled receptor, as a protein that can cross the membrane seven times, has a very complex molecular structure, and even if humans study it, it is also the reason why it hinders human research.

Bovine rhodopsin protein is the most stable and clearest seven-pass transmembrane protein found by scientists.

At least at this stage, it is true.

Of course, the ambitions of scientists are limitless, and they will always want better materials.

Yang Rui also wants that when his laboratory becomes the world's top laboratory, even if it spends twice or three times the funding, he will also explore such a world.

In China in 1986, the ion channel laboratory of Peking University was not qualified to do so.

Ordinary laboratories choose one kind of material, or in order to spread the risk, choose a variety of experimental materials, and directly carry out the experiment is the common practice.

Yang Rui gave his confidence to bovine rhodopsin protein, and that's it.

This kind of knowledge is taken for granted to Yang Rui, but to Su Xiankai and others, it is a little strange. After 30 years, even if domestic graduate students do not read relevant papers, they can learn a lot of relevant information from their classmates and teachers. But in China in 1986, there was no such condition.

Ordinary scholars in China have no idea about how the top foreign laboratories do experiments and how the top foreign laboratories do scientific research.

Not to mention laboratory management, it is all confidential.

Yang Rui knows very well that in the field of g-protein coupled receptors, everything is chaotic. This was true in 1986 and the same was true in 2016.

It's just that, equivalent to other people, Yang Rui can see a little more information.

For example, in the chaotic vision, find the most appropriate material.

"I'll let someone send a batch of bull's eyes." Yang Rui stood up, made a call, and hung up after a few polite sentences: "That's it, Lao Su, let's double the size, it's okay." "

"Double?" Su Xiankai said somewhat uncomfortable: "Should we take it step by step and increase it by about 20% first?"

Yang Rui sighed and said: "Doubled, it's coming step by step."

He glanced at Gu Qiang and Fan Zhenlong, and said: "Don't listen to them saying that it has doubled the expectation. It is true. Based on your current progress, I think that if you provide twice as much bovine rhodopsin protein, it is far away. Far from enough."

Gu Qiang was unhappy, and said, "Director Yang, you look down on people."

Yang Rui raised his eyebrows and asked, "Say something that makes me stand out, which step have you achieved now?"

Gu Qiang hummed for a long time, and said, "We are close."

"If it wasn't for you to make some snacks, do you think I would add materials to you?" Yang Rui half-heartedly gave a sentence, and said: "Don't be wordy, you have to learn your lesson too, don't let the old man Sue’s hard work was in vain."

Su Xiankai's face had already collapsed and said, "I think I'm almost like a coolie, Director, how can I say it's also a deputy team leader? Can't we give me a more energetic job?"

"Your job is vigorous enough. In addition, our main job recently is to produce bovine rhodopsin protein. Therefore, your side is the main direction of attack..."

"You lied to me." Su Xiankai was full of suspicion.

Yang Rui solemnly said: "In the next month or two months, the place where funds, materials, equipment and personnel will be greatly increased is the production of bovine rhodopsin protein, understand?"

Su Xiankai immediately believed it, but frowned and asked, "Why?"

"Why? Because your teammates have a low level of experimentation, are slow to do, and consume a lot of money. If you don't have other good ideas, you can only feed them with materials."

Yang Rui's succession of aggressive generals finally aroused Gu Qiang.

Comrade Gu Qiang came up with the case and said: "You are optimistic, we don't need to feed, and the next day, I will increase the success rate."

"Yes, I can speak Beijing dialect. Erhua sounds decent." A smile suddenly appeared on Yang Rui's face, which caught people off guard.

Gu Qiang was choked and couldn't talk about it.

Xu Zhengping coughed at this time and asked, "Yang Rui, really want to double the production of bovine rhodopsin protein?"

"Yes, this is the only chance for us to catch up with foreign laboratories." Yang Rui deliberately said something serious, and then said: "We must realize the strength of the enemy, and we must not underestimate ourselves. Your experiment success rate is indeed not good. High, but what about the researchers in foreign laboratories? How many times can they succeed? It’s not a thing. This is a big project, and the next experiment will be a lot and it will be very long. We have to gain the advantage from the source so that we can eliminate it. Other forces of the enemy, right?"

"For foreign laboratories, don't you want to increase the success rate from the source?" Fan Zhenlong asked.

"First, foreign laboratories cannot determine that the most appropriate material is bovine rhodopsin protein. Second, even if they realize it, they also hope for the success rate of the next experiment. Third, they obtain fresh cattle. The channels of the eyes can't keep up with us."

The last one is half-truth and half-hypothesis by Yang Rui.

To a certain extent, after Yang Rui established a genetic engineering laboratory, his relationship with various animal husbandry companies can provide him with a large amount of animal materials, limited to such a small point, which is the world's top condition.

When Fan Zhenlong heard Yang Rui's second point, he was a little stunned. He knew Yang Rui's speaking habits too well.

Fan Zhenlong couldn't help asking suspiciously: "What do you mean by the success rate of the next experiment that they are hoping for?"

"It means literally." Yang Rui coughed.

"No, you say hope, you mean there should be no hope."

Yang Rui laughed twice without answering directly.

Fan Zhenlong asked again and again.

Yang Rui considered the language, and said, "Let's put it this way, chances are greater."

More than accidental, the three-dimensional structure of g protein-coupled receptors, like various starry sky exploration projects, is completely piled up by numbers.

What amount is used to pile it up?

Bovine rhodopsin protein!

At this point, the top foreign laboratory, UU Reading www.uukanshu.com, has actually ignited and fallen behind.

Only when they realize that bovine rhodopsin protein is the best material, and only when they realize that a large amount of material is needed to pile up the three-dimensional structure of the g protein-coupled receptor, can they be regarded as catching up with the progress of the ion channel laboratory. .

Of course, once the top foreign laboratories realize this, they can catch up very quickly.

Yang Rui can only run as fast as possible.

Fan Zhenlong was completely shocked by Yang Rui's answer.

He hesitated for a long time and asked, "How accidental?"

"Don't worry, I will organize the production of enough materials." Yang Rui patted Fan Zhenlong on the shoulder, letting him relax.

Fan Zhenlong's heart is about to explode!