Judging from Wang Liangcai's eyes, Yang Rui's operation is hard to say.

He is a student from Tong's line. He studied genetics in a serious way. He also trained with scholars from the Institute of Hydrology of the Chinese Academy of Sciences for many years. After that, he taught and researched at the University of Science and Technology of China. Kind of items. As far as the egg stripping technique is concerned, Wang Liang is world-class, far surpassing the so-called egg stripping master of Peking University, and Yang Rui's technique is not in his eyes.

However, Wang Liangcai wanted to remember every movement Yang Rui operated at this time.

In just a few tens of minutes-almost equivalent to the time spent waking up from watching Wong Kar Wai's movie in a movie theater, Yang Rui has basically completed the core removal operation.

Denuclearization is not difficult, especially for researchers from the Fisheries Institute.

But Yang Rui only failed once and successfully completed the de-nuclear operation, which is truly surprising.

In other words, Yang Rui's operation method is not worth mentioning, but he used such an operation to easily complete the denuclear operation, which is the reason why Wang Liangcai is concerned.

In the cloned cell operation, a total of two enucleation operations are required.

The first enucleation operation is to take out the nucleus of the oocyte of the sheep. Because this nucleus is not used in the later stage, the first point of the enucleation operation is to **** out the cytoplasm of the cell as little as possible, and at the same time, to **** the nucleus cleanly.

The second enucleation operation is for ordinary cells.

The cloned sheep uses sheep's mammary gland cells. The main point of operation at this time is to completely **** out the cytoplasm of the sheep's mammary cells. As for the mammary cells themselves, it does not matter because they are also discarded in the later stage.

In general, both steps actually require refinement, and there are few shortcomings that can be tolerated. The best condition is to completely separate the nucleus from the cell itself.

Otherwise, if the nucleus left by the oocyte is involved in the development later, it is hard to say whose sheep is born.

If it is used for cloning, you originally wanted to clone the sheep Dolly, but as a result Dolly carried Domey's genetic gene. This kind of cloning is not clear.

It's not a sensational clone anymore.

From the very beginning, Wang Liangcai was very clear about the purpose of this part of the operation, but in order to **** up the nucleus of the oocyte, he had to **** out more cytoplasm. As a result, the remaining cytoplasm was not enough to develop and it failed.

The same is true of Gu Qiang's operation. In this step, the cytoplasm is too easily sucked out.

On the contrary, it is the second step. It doesn't matter how much cytoplasm is lost. It only requires the integrity of the nucleus, which lowers the technical requirements of the experimenter.

Yang Rui's newly developed technology is different.

Although the goal of the operation has not changed, it is still to aspirate all the nuclei and as little cytoplasm as possible.

However, Yang Rui's operation lowered the requirements a little. Not only did he not seek to completely **** out the cell nucleus, but instead deliberately sucked up the cell nucleus, and then sucked out the fragments little by little.

It is possible to completely **** out the nucleus, but it is too difficult.

Moreover, the nucleus is not a hard solid, it is more like a balloon swimming in a viscous liquid. If it is completely sucked out, it will inevitably increase the suction force, thereby sucking out more cytoplasm. In addition, When the balloon comes out, it always brings out more cytoplasm. Sometimes it brings out more, and it directly declares that the experiment has failed. Sometimes it brings out less and has a lot of luck.

If you **** it through, of course, it will cause more trouble.

Imagine that a balloon is broken in the liquid, and the gas inside the balloon is still floating out. Bubbles are formed in the liquid. It is so troublesome to **** them out one by one.

However, trouble is indeed troublesome, but it is a troublesome task that can be completed. In addition to careful and tedious operations, sucking debris is much easier than sucking a whole balloon.

Sometimes, if the nucleus needle is well controlled, it doesn't even need to **** a lot of debris, and the task will be completed after three or five times of negative pressure suction.

After Wang Liang discovered this, he could no longer walk.

Immediately afterwards, Wang Liang discovered that the suction was only the smallest of the new technologies developed by Yang Rui.

Using the spindle to control the amount of absorption is the most powerful, it can be said to be ingenious.

As the name suggests, the nucleus needle is used to **** the nucleus. How small is its tip? Basically, you may not be able to see clearly under a microscope with normal magnification.

Therefore, when the nucleus needle is operated, a slight vibration will cause it to swing violently.

You can imagine the feeling of holding a rope in your hand when threading a needle. The cell is much smaller than the eye of a needle, and the hand trembles a lot, let alone sucking the nucleus, it is normal to pierce the cell.

Yang Rui's level was weaker than Wang Liang's. He was an experimenter with a certain level. He still accidentally punctured an oocyte, and the difficulty can be measured.

However, when the nucleus needle is against the spindle, the vibration disappears naturally.

It's like holding a long stick in your hand, the head of the stick will tremble, and when you poke the stick against the wall, the head will not tremble.

In addition to the ability to control the shaking of the nucleus needle and increase accuracy, another advantage is that it controls the back and forth vibration of the nucleus needle.

That is to say, when the nucleus suction needle pierces the spindle, it seems to have added a fault-tolerant device out of thin air, making the operation easier at once.

It's easy to say, but it's not easy to think of using this trick.

The most difficult part is that you find the correct location for the poke.

The spindle is also a very important part of the cell, and you can't poke it if you want to. It is inherently tougher, but some parts are too tough to hinder absorption, and some parts are unexpectedly fragile and easy to puncture.

The rest of the spindle that can be pierced, it is best to be in a position that is easier to operate.

Wang Liangcai watched Yang Rui's movements, thinking about it, wishing to master the new method immediately and try it later.

At this point, Yang Rui could not meet Wang Liangcai's request.

What he did was slow, but he only tried the new method a few times. The awkward operation makes people want to jump, just like a kid who just learned to shoot a three-step layup. It's ugly when he doesn't foul. To die, not to mention that there are more fouls than walking.

"Professor Yang, I understand a bit, or else, let me try it?" Wang Liangcai whispered.

Yang Rui lifted his eyelids and said, "Why, do you think my operation is not good?"

Wang Liangcai cleared his cough and said against his will: "How can you do it?"

"I also think, wait a minute, I will finish this de-core." Yang Rui really feels that what he did is quite good. It is not as good as Wang Liangcai's operation, and it is still an improvement compared to the previous operation.

Naturally, he ignored the difficulty gap between the old technology and the new technology.

Wang Liang was so anxious that he couldn't urge him, so he could only watch it obediently.

If Wang Liangcai had added two pounds of bear cell nuclei, he would have jumped up to tell the truth. The same conditions apply to other researchers in the laboratory.

As time went by, Su Fan, Gu Qiang, Tian Bing and others gathered in front of the experimental platform in silence.

Everyone knows how Yang Rui's experimental ability is.

Everyone knows how long an oocyte can withstand the ravages.

Normally, if Yang Rui is given an oocyte, he will not be able to play for two hours without puncturing it.

After a while, the progress of the experiment will get deeper.

Think of Gu Qiang's luck to achieve the nuclear injection. Wang Liang slept in the laboratory for several weeks before barely catching up with the progress... Yang Rui can't just take Gu Qiang's luck and just do it for a few hours in one go.

The more researchers concerned, the more curiosity of other researchers.

In the afternoon, people around the experimental platform where Yang Rui was located were already crowded.

Yang Rui felt nothing.

Unlike the boring experimentation of other people, he was trying new methods, it was a bit like playing a new game ~lightnovelpub.net~ at first, he couldn't stop.

Gurgling.

Someone's stomach rang.

Yang Rui raised his head blankly and rubbed his sore neck, only to notice the strange gazes around him and the moving heads.

"Hey, don't get crowded, isn't it hot?" Yang Rui reluctantly drove everyone away: "Pay attention to all, don't pollute the experimental platform."

Everyone quickly took two steps back to make room.

"It's almost eight o'clock. If you haven't eaten, go to eat. I have to have several hours here." Yang Rui touched his stomach and decided to wait before eating. The stomach can wait, but the cells can't wait.

Everyone nodded their heads and persuaded each other: "Lao Tian, ​​you have a bad stomach, go eat quickly."

"Xiao Lei, stop relying, go eat and eat."

"Su Fan, today I have your favorite braised pork with braised pork, fast hands and slow hands."

"Then why don't you go?" Su Fan curled his lips, not paying attention to the persuasion of others.

In fact, no one wants to leave at all.

How rare is the opportunity to see the demonstration of new technologies. It was the Nobel Prize-winning Yang Rui who was demonstrating. In the future, he said, "When I saw Professor Yang doing the denucleation of egg cells," he naturally gained his style.

Yang Rui didn't say much. He was just demonstrating technology, so he couldn't drive people away.

He touched his stomach and continued to work, and the others gathered together as usual, watching and discussing in a low voice.

In a laboratory, there are always high and low levels, especially when facing a specific subject, there are always people who have a thorough grasp, and there are always people who are not good at it.

At this time, everyone's focus is different.

The so-called layman watching the excitement and the insider watching the doorway are the same in the same laboratory.

Even just looking at everyone's notebooks, Wang Liangcai remembered more than others.