Yang Rui took out a Montblanc from the drawer. Before writing down, he patted his head and said, "You should make a copy first, so that I won't be messed up."

"It's okay, you can draw whatever you want." Gu Qiang's attitude is extremely well-behaved.

"It's better to make a copy and keep a foundation, so that you can compare it later." Yang Rui insisted.

Gu Qiang had to make copies according to his words, looking at the hundreds of pages of experimental design, a little reluctant. Not reluctant to write the experimental design, but reluctant to copy paper and ink.

Today's photocopiers are all Japanese products, not to mention the high prices of the machines, and the consumables are even more expensive. When Yang Rui worked on test papers at school, he used to organize mimeographs.

Common materials are copied by everyone, and try not to waste copy paper and other consumables.

At this moment, naturally I can't wait to copy.

Gu Qiang hardened his scalp and printed a copy of the experimental design he had written, and when he returned it to Yang Rui, he said, "In the future, I will find someone to copy these things. It is too expensive to print. The paper is only a few cents. of."

"It's not expensive to find someone?"

"For work-study students, 10 yuan a month is enough." Gu Qiang said naturally.

Ten yuan is a lot, and rural families in many places now earn less than 120 yuan a year in cash.

If a student eats in the cafeteria, 10 yuan can guarantee a full stomach, with a little bit of meat.

Yang Rui ignored this stubbornness. He looked down at the experimental design of Gu Qiang's printing plate, took off his pen cap while talking, and said, "I need to save a little office expenses. Let Director Xu worry about it. Your time is up to you. Don't waste it on this. The laboratory wants you to produce it, not to save it."

"What is saved is output." Gu Qiang whispered.

"If you can complete the topic of in vitro culture within two months, it will be the real output." Yang Rui's red pen was drawn on Gu Qiang's experimental design, and the first line circled three circles. .

Gu Qiang was shocked by the red mark, and was shocked by what Yang Rui said. He couldn't help repeating: "Two months?"

"The progress of Wang Liangcai's research group will also be accelerated." Yang Rui didn't say why, everything was said without saying.

The corner of Gu Qiang's mouth twitched twice, knowing that Yang Rui must have also been involved in Wang Liangcai's research.

Come to think of it, it is impossible for Yang Rui not to intervene.

Since the beginning of the cloning project, Wang Liangcai and the Gu Qiang team have been busy competing and experimenting, and Yang Rui has not stopped thinking.

Now that he speaks out the results of his considerations, it will naturally help improve the progress of the project.

However, in two months, it is necessary to develop the subject of in vitro culture from scratch, but Gu Qiang is not confident.

"I thought I would have more time." Gu Qiang said cowardly.

Yang Rui smiled, picked up the pen again, and began to draw a red circle.

On the first page alone, he drew more than a dozen red circles, almost completely unrecognizable.

Gu Qiang took a breath to himself, then lowered his head to look at the place Yang Rui marked out.

The term "in vitro fertilization embryo" was circled and crossed, and the oxygen consumption next to it was circled without any other changes.

Further on, Gu Qiang saw the circle that Yang Rui drew on the term "densification" and the circle on the "culture solution".

Gu Qiang finally knew why Yang Rui asked himself to make a copy.

In this way, the entire experimental design is completely changed.

If you don't leave a bottom plate, I really don't know the specific content of the original.

"I hope you can focus your research on embryos after the blastocyst stage. However, the points you need to pay attention to are not only that." Yang Rui saw that Gu Qiang was watching, and he didn't seem to be hit too much. Apart from drawing circles and crosses, I gave an explanation by the way, saying: "You want to refer to the culture system of in vitro fertilization embryos for the cultivation of cloned embryos. I do not approve.

Yang Rui clicked the pen twice on the red cross and said, "Of course, the judgment of the frontline researchers is the first criterion, but from my personal understanding, the original system is used directly without analysis. The result is likely not to save time, but to waste energy."

"Do you think cloned embryos will be different from normal embryos?" Gu Qiang was a little surprised.

Regardless of theological things, scientists have a clear understanding of embryos and other reproductive bodies. They believe that cloned embryos should be the same as normal embryos. Otherwise, why does cloning cause so much controversy.

Therefore, in the process of in vitro culture, Gu Qiang naturally adopted the same technical solutions as "test tube baby" and "test tube cow".

Yang Rui put the pen aside and said, "I don't think that cloned embryos will be essentially different from normal embryos, but they are of course different. First of all, the cloned embryos we got are using very little cytoplasm. The oocytes developed out of the oocytes are congenital malformations. Secondly, the oocytes have been soaked in a variety of operating fluids. For example, as we currently know, the soaking of sucrose solution will have adverse effects..."

Yang Rui's voice was steady and said: "In short, cloned embryos are abnormal embryos. If you use normal embryo culture methods, problems are likely to occur."

He said it was a guess, but in fact, Yang Rui was sure that it would go wrong.

This is also a difficulty in in vitro culture.

More problems will appear with poorly developed oocytes.

In short, in order to replace the nucleus of the egg cell, the operation of the scientist has caused great damage to it, and the in vitro culture is to make up for this damage.

For example, if the weight of 5 kg is regarded as the minimum standard weight of a newborn, 2.7 Newtons at birth will not be easy to survive. The modified cloned oocyte, which is approximately equivalent to a child weighing 0.5 kg, will never survive under natural conditions.

To allow it to develop normally, it is no longer the problem of whether to feed Sanlu milk or not to buy imported milk powder.

Of course, if you can't afford imported reagents, the oocytes will not survive.

Gu Qiang was shocked by Yang Rui's detailed explanation, and couldn't help asking in a low voice: "Have you done in vitro culture projects before?"

"We did some preparatory research when we were doing embryo transfer of cattle." Yang Rui said.

Gu Qiang was full of suspicion. Although the embryo transfer of cattle also carried the word embryo, it was an order of magnitude different from the problem facing now.

Not to mention, the funds for in vitro culture of cloned sheep would have to be spent in the millions. Basically all the remaining resources of the Gu Qiang team had to be transferred.

What about embryo transfer of cattle? Even with the hidden investment of companies such as China Animal Husbandry, it doesn't add up to that much.

Gu Qiang licked his lips and asked, "When you do a cow embryo transfer, you want to clone it."

Yang Rui glanced at Gu Qiang with a smile, and did not answer. He used his pen to point out the experimental design, and said: "Next, it is the experimental details. I have some ideas for your reference?"

"You said." Gu Qiang's attitude is positive.

"From the data point of view ~lightnovelpub.net~ adding various additives to the basic nutrient solution should also be able to improve the development of cloned embryos." Yang Rui did not elaborate on the information, and said: "We are from cell embryos When we start to cultivate, we will face the interruption of embryo development."

Blocked embryonic development refers to the phenomenon that the embryo develops to the four-cell stage and stops its development. This is mainly related to the activation of the embryonic genome.

There is no genome project now. To break this block, you have to do many different attempts.

Yang Rui did not elaborate, but in Gu Qiang’s experimental design, he drew two circles and said: “Adding fallopian tube fluid to the culture medium and co-cultivating fallopian tube epithelial cells. I prefer these two solutions. You You can try it first."

"Okay." Gu Qiang nodded repeatedly.

"The culture medium is also an important point. You have to make experimental records, complete experimental records." Yang Rui reminded specially.

A clear formula is very important for the culture medium.

Of course, today's scientists have not realized this, because no one has started cloning yet.

In the later generations, if the cloning part is the most complicated and the most difficult part, it is not the technique of egg stripping, but the preparation of embryo culture medium.

Yang Rui thought for a while, and decided to help Gu Qiang reduce the burden a little, and said: "From my personal feeling, I think we have used fetal bovine serum as a more promising additive for culture medium. Epidermal growth factor This is a new thing and should be tried. Insulin growth factor, antioxidants, anthocyanins, I think they can all be considered. In addition, oxygen is also very important."

"Okay." Gu Qiang recorded it purely, without any clues for the time being.

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