The shotgun method is not the best gene sequencing method.

Its accuracy is very poor, so its another translated name is shotgun.

Don't count on where to hit, it's a feature of a big shot.

In the eyes of the most traditional biologists, this method is actually not very popular. Because it goes against intuition, accuracy is also very problematic.

Sanger sequencing is the sequencing of one gene per gene, and it is completely in accordance with the sequence of gene arrangement. The advantage of this is that by completing the sequencing of a gene, your map will be a little complete.

The Human Genome Project is based on this. Watson and others imagined using 3 billion U.S. dollars, an average price of one dollar per human gene, to create a human genome map.

Of course, they also know that with the current difficulty of sequencing, one dollar for sequencing one gene is not enough, but is it flickering anyway? Taking into account the dividends of technological advancement, plus the cost savings of various costs, plus additional later The cost of it almost seems to be achievable.

It just can't be done-Isn't Dalbecco's cancer conquering plan incomplete? If you don't finish it, you won't finish it.

Compared with the Sanger sequencing method that takes one step at a time, the technical characteristics of the shotgun method are actually more prominent, but it is also more troublesome.

The shotgun technique is a single shot, breaking a gene chain into pieces.

Just like a dog chain, when the shotgun goes out, it is naturally disassembled into metal rings, and then the ATCG of the metal ring is measured in one breath.

Up to this step, it is very simple, it can be said to be a shot.

The next step is the most disliked step by Dalbecco and others.

You need to rearrange the metal rings in the original order to form a dog chain.

This step is very difficult, so in history, the shotgun method was originally used for microbial sequencing. Because the genes of microorganisms are simple, they are arranged well.

After changing the human genome, sorting by shotgun method is much more difficult than a jigsaw puzzle.

unless--

"We use computers to solve the problem of out-of-order shotgun methods." Standing in Huarui's laboratory, Yang Rui stated the direction of the experiment without even thinking about it.

Huarui Laboratory is his private laboratory, all rights are in his hands, and there is no need to worry about it.

However, Huang Mao said with some worry: "It will cost a lot of money to do the human genome project, can you make it back?"

Yang Rui laughed happily and said, "When did you care about funding?"

"I'm not afraid that your company will go bankrupt." Huang Mao said naturally: "I heard that the bankruptcy law has already been implemented. Insolvent companies are going to go bankrupt."

In the first two years, several state-owned enterprises were forcibly closed down in China, and they were widely reported. They were the only ones that went bankrupt in the 1980s.

"We are far from repaying our insolvency, so you can rest assured."

"I want to rest assured, I'm afraid that you will not be able to keep up with the follow-up funds." Huang Mao said casually.

"There will always be funds when you need it. Let's start doing it first." Yang Rui thought in his mind that he had hundreds of courtyards, and he had just bought more than 20 buildings in a commercial complex, one house and one house of antiques. Tea and stamps always feel that they are rich and oily.

He is of course rich and wealthy, by the standard 30 years later.

But at the end of the 1980s, although the houses in the capital were still out of reach for ordinary people, they were not enough for scientific research. Even if it is the sale of the commodity community that has just been bought, that is, the daily expenses of the Huarui Lab for several years. The bottomless pit like the Human Genome Project can be filled without even thinking about it.

If you think so, you have to thank the soaring house prices. In fact, later generations of research institutes actually have a lot of dormitories for distribution. Researchers’ property is mainly concentrated in their own real estate. Their asset appreciation speed really exceeds the appreciation speed of technological development...Theoretically, If some large research institutes sell all the land, they might really be able to study the human genome project by themselves...

Yang Rui’s thoughts cannot be known to Huang Mao. The latter listened to Yang Rui’s words and shook his head slightly, and said, “It’s okay to listen to you, but you have to pay attention to it. For the pre-research of the Human Genome Project, I can I don’t see any possibility of returning funds."

The pre-research is purely basic science, and when it comes to giving back funds, it is really rare.

If it were not for the later Salalah Gene Corporation, to be honest, Yang Rui would not be sure that the shotgun technique could sell for money.

In fact, Salalah Genetics has not really succeeded. Its leader, madman Venter, despite flicking $300 million, developed the shotgun method and used it in the Human Genome Project, and tried to register human genes as patents-if he succeeded. If so, any targeted drug in later generations may be outrageously expensive.

Salalah will probably be really rich.

Unfortunately, Comrade Venter did not succeed.

So, speaking of it, the Human Genome Project really has no history of making money.

The pharmaceutical companies that made money later did not give the project team of the Human Genome Project a penny in return.

However, the fact that Americans cannot make money does not mean that the Chinese cannot make money.

At this time, the benefits of independence are reflected.

As far as Yang Rui's current status is concerned, when such cutting-edge technology related to biology and no existing laws can be used, the formulation of new laws and regulations is almost his decision.

GMP committees and everything are of the same nature.

Don't let a top scientist like Yang Rui make judgments, shouldn't you find a liberal arts student to guess?

Many people think that cloning is really a copy of a person.

In fact, the difference between human clones and test-tube babies is only at the genetic level. They can still be pregnant in October, give birth naturally or be delivered by caesarean section, and then be educated like normal children-if you want to get the same Hitler or Einstein, It depends on whether the parents have this ability!

Having the qualifications to participate in the formulation of laws, this is the hidden power of top scientists.

From the perspective of one's needs, why are so many people willing to immerse themselves in the painful and boring world of science? Some of them really like the scientific world, while others use this as a springboard to enter the power class in one breath.

For example, some famous economists in the 1980s and 1990s played a bigger role than many ministers and truly changed China.

People who are suffering in stocks, happy for stocks, and frustrated by stocks, are hard to imagine that this stock market depends on the reports of several people for as long as twenty years.

Similarly, people who suffer from high blood pressure, suffer from rheumatism, suffer from bone hyperplasia, and suffer from tracheitis and out of breath, if a few top biologists are willing to turn to, it can be more. Living for a few years will make life much easier, just like those diseases that are valued, such as diabetes and AIDS. The living conditions of patients in the 1980s, 1990s, 00s and 10s were completely different.

**The life of patients with dysfunction ~lightnovelpub.net~ is completely changed by the little blue pill.

Before the blue pill, many men’s unspeakable concealment was really unspeakable and could not be saved. After the blue pill appeared, most men’s embarrassment was that the hairline gradually changed. I can't hide it.

"Financial issues should not be ignored. Well, if you are really worried, let's start another group and do antihypertensive drugs." Yang Rui is undoubtedly ready to pull Viagra out.

The Human Genome Project is a costly project. Although Professor Cai promised to help find money, Yang Rui couldn't relax himself.

As he said, the allocation is determined by the proportion of funds, and he still needs some effort to ensure control.

If you find something that costs less and is quick and effective, you will naturally think of Viagra.

Of course, the main ingredients of Viagra today are still being tested as antihypertensive drugs.

Huang Mao didn't doubt that he was there, and only said: "Prescribing a set of antihypertensive drugs at the same time, it will be too busy."

Yang Rui has made too many new medicines, and every time he makes a lot of money, it is not surprising that he makes medicine to make money. He is only worried that Huarui's scale cannot support the two projects.

Yang Rui shrugged and said: "I am more interested in the current discussion of nitric oxide, and I am going to buy a compound to do it."

"Huh, shall we buy the compound?"

"Well, I will find someone to buy it, so don't worry."

Huang Mao smiled bitterly: "Why don't I worry about it. We used to make active compounds and then sell them to pharmaceutical companies for clinical trials? We don't have experience in clinical trials..."

"It's the first time for everything." Yang Rui said with rich wealth: "We have money, don't worry."

Huang Mao was even more worried, for fear that Yang Rui would have lost all his funds and bought back the compound that had no use for eggs.